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mts 492nm  (Promega)
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Promega mts 492nm
Vehicle control (EtOH) or 4-OHT or was added to Mdm2fl/flp53−/− lymphomas expressing CreERT2 and GFP or GFP alone. A–F) PCR Mdm2 gene rearrangement <t>(A),</t> <t>proliferation</t> <t>(MTS</t> assay, quadruplicate; B), cell number (C), viability (D), sub-G1 DNA (E), and Annexin-V (F) measured (C–F, triplicates). G) Western blotting 16hrs after EtOH or 4-OHT addition; cleaved Caspase-3 (CC3). H, I) Cell cycle (H; representative histograms; values in inset, left; G2/M mean values, right) and phospho-histone H3 (I) 12hrs after EtOH or 4-OHT (triplicate); colcemid (Col). B, *p<0.0002; C, *p=0.011, **p<0.0002; D, *p<0.0002; E, *p<0.00009; F, *p<0.006; H, *p=0.00102.
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Vehicle control (EtOH) or 4-OHT or was added to Mdm2fl/flp53−/− lymphomas expressing CreERT2 and GFP or GFP alone. A–F) PCR Mdm2 gene rearrangement (A), proliferation (MTS assay, quadruplicate; B), cell number (C), viability (D), sub-G1 DNA (E), and Annexin-V (F) measured (C–F, triplicates). G) Western blotting 16hrs after EtOH or 4-OHT addition; cleaved Caspase-3 (CC3). H, I) Cell cycle (H; representative histograms; values in inset, left; G2/M mean values, right) and phospho-histone H3 (I) 12hrs after EtOH or 4-OHT (triplicate); colcemid (Col). B, *p<0.0002; C, *p=0.011, **p<0.0002; D, *p<0.0002; E, *p<0.00009; F, *p<0.006; H, *p=0.00102.

Journal: Cancer research

Article Title: Mdm2 is required for survival and growth of p53-deficient cancer cells

doi: 10.1158/0008-5472.CAN-17-0809

Figure Lengend Snippet: Vehicle control (EtOH) or 4-OHT or was added to Mdm2fl/flp53−/− lymphomas expressing CreERT2 and GFP or GFP alone. A–F) PCR Mdm2 gene rearrangement (A), proliferation (MTS assay, quadruplicate; B), cell number (C), viability (D), sub-G1 DNA (E), and Annexin-V (F) measured (C–F, triplicates). G) Western blotting 16hrs after EtOH or 4-OHT addition; cleaved Caspase-3 (CC3). H, I) Cell cycle (H; representative histograms; values in inset, left; G2/M mean values, right) and phospho-histone H3 (I) 12hrs after EtOH or 4-OHT (triplicate); colcemid (Col). B, *p<0.0002; C, *p=0.011, **p<0.0002; D, *p<0.0002; E, *p<0.00009; F, *p<0.006; H, *p=0.00102.

Article Snippet: Proliferation determined by MTS (492nm; Promega) or MTT (562nm; Sigma) assays (quadruplicate), as we previously reported ( 24 ), ( 25 ).

Techniques: Control, Expressing, MTS Assay, Western Blot